The Electron Microscopy Laboratory is located in the basement of the McArdle Laboratory for Cancer Research. This laboratory has existed and provided services to McArdle investigators and other Medical School staff for 30 years. Dr. Henry C. Pitot has been and continues to be the faculty advisor for this facility.
This laboratory performs the following services:
- Initial consultation on the feasibility of a proposed project
- Tissue preparation: Fixations, staining, embedding, and sectioning with an ultramicrotome and staining of grids
- Collaborative preembedding immunocytochemistry projects
- Observation and photographic film recording of images on the Hitachi H-7000 electron microscope
- Developing of film, and printing of micrographs
- Consultation on the obtained images in the micrographs
Available equipment at the facility include:
- Hitachi H-7000 transmission electron microscope (under service contract)
- Reichert Ultracut E ultramicrotome
- Denton Vacuum DV-502 with glow discharge accessory
- Ilford 2150RC print processor
- 4 X 5 Simmons Omega photographic enlarger
- Fume hood, oven, and other lab equipment needed in the preparation of tissues and cultures for EM
Contact Information
Dr. Henry C. Pitot, Faculty Advisor
pitot@oncology.wisc.edu
Dr. Carol A. Sattler
sattlerc@oncology.wisc.edu
(608) 262-4545
Location
McArdle Laboratory for Cancer Research
1400 University Avenue
Madison, WI 53706
Rooms: B21/B19
Hours
6:30 AM to 2:30 PM (or as experiment dictates)
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